Comparison of neuraminidases of the same subtype but from different species using a new method of titration
Identifieur interne : 000303 ( France/Analysis ); précédent : 000302; suivant : 000304Comparison of neuraminidases of the same subtype but from different species using a new method of titration
Auteurs : B. Fiszon [France] ; C. Hannoun [France]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 1990.
English descriptors
- Teeft :
- Antigenic, Antigenic variation, Avian, Avian strains, Avian viruses, Blood cells, Different species, Duck strains, Hemagglutinin, Hlnl, Human neuraminidases, Human strains, Influenza, Influenza virus, Influenza viruses, Last dilution, Last virus, Method table, Neuraminidase, Neuraminidase activity, Neuraminidases, Pasteur institute, Peanut hemagglutinin, Same subtype, Second group, Sialidase activity, Surface glycoproteins, Titration, Viral, Viral ecology, Viral suspension, Virus sera.
Abstract
Abstract: Neuraminidase is one of the two surface glycoproteins of influenza virions. In order to compare neuraminidases of the same subtype but isolated from different species (man, birds, pig), a new and simple method was adapted and optimized using peanut hemagglutinin. Results were very similar to those obtained with the classical method recommended by the WHO, using fetuin as a substrate. The technique was used to examine the relationship between animal and human neuraminidases belonging to serotypes N1 and N2. The resulte confirm the possible role of ducks as a reservoir for influenza viruses and the eventuality of interspecific exchanges.
Url:
DOI: 10.1016/0166-0934(90)90148-9
Affiliations:
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<term>Different species</term>
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<term>Pasteur institute</term>
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<term>Same subtype</term>
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<front><div type="abstract" xml:lang="en">Abstract: Neuraminidase is one of the two surface glycoproteins of influenza virions. In order to compare neuraminidases of the same subtype but isolated from different species (man, birds, pig), a new and simple method was adapted and optimized using peanut hemagglutinin. Results were very similar to those obtained with the classical method recommended by the WHO, using fetuin as a substrate. The technique was used to examine the relationship between animal and human neuraminidases belonging to serotypes N1 and N2. The resulte confirm the possible role of ducks as a reservoir for influenza viruses and the eventuality of interspecific exchanges.</div>
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